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. 2019 Nov 26;10:2749. doi: 10.3389/fimmu.2019.02749

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Copyright © 2019 Agallou, Athanasiou, Kammona, Tastsoglou, Hatzigeorgiou, Kiparissides and Karagouni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dendritic cell induced-maturation after CPA_160−189-loaded p8-PLGA nanoparticles. Bone-marrow-derived DCs were exposed to CPA_160−189-loaded p8-PLGA nanoparticles for 24 h. DCs cultured in medium alone or p8-PLGA nanoparticles served as controls. Nanoparticle uptake and DC maturation were assessed by flow cytometry. (A) Evaluation of CPA_160−189-loaded p8-PLGA nanoparticle uptake by DCs. Percentage of (B) CD40-, (C) CD80-, (D) MHCI-, and (E) MHCII-expressing CD11c⁺ DCs. Mean fluorescent intensity (MFI) of (F) CD40, (G) CD80, (H) MHCI, and (I) MHCII on CD11c⁺ DCs. Results represented as mean ± SD of three independent experiments and levels of significance, indicated by p-values, were assessed by one-way ANOVA and Tukey's multiple comparison tests (*p < 0.05; **p < 0.01; ***p < 0.001).