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. 2019 Nov 26;10:2749. doi: 10.3389/fimmu.2019.02749

Figure 3.

Figure 3

Protective efficacy of CPA160−189-loaded p8-PLGA nanoparticles in BALB/c mice against L. infantum. (A) BALB/c mice were vaccinated thrice with 2 week intervals with CPA160−189-loaded p8-PLGA nanoparticles or p8-PLGA nanoparticles and then were challenged with L. infantum promastigotes. Parasite load was assessed at 4 and 16 weeks post challenge in liver (B,D) and spleen (C,E) of vaccinated and non-vaccinated mice by qPCR. Each point depicts the data from a single animal (n = 5 per group). Results are shown as mean ± SD and levels of significance, indicated by p-values, were assessed by one-way ANOVA and Tukey's multiple comparison tests (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Experiments were conducted twice with similar results and representative results from one experiment are given.