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. 2019 Nov 26;10:2749. doi: 10.3389/fimmu.2019.02749

Figure 4.

Figure 4

CPA160−189-specific and parasite-specific immune responses in vaccinated BALB/c mice 4 and 16 weeks post challenge with L. infantum. BALB/c mice were vaccinated thrice with 2 week intervals with CPA160−189-loaded p8-PLGA nanoparticles or p8-PLGA nanoparticles and then were challenged with L. infantum promastigotes. At 4 and 16 weeks post challenge, spleen cells were collected and splenocytes were stimulated in vitro with CPA160−189 or SLA for 72 h. The culture supernatants were assayed for CPA160−189-specific (A) IFN-γ, (B) TNFα, (C) IL-4, and (D) IL-10 and parasite (SLA)-specific (E) IFN-γ, (F) TNFα, (G) IL-4, and (H) IL-10 production by Luminex. Each point depicts the data from a single sample (n = 5 per group). Results are shown as mean ± SD and levels of significance, indicated by p-values, were assessed by one-way ANOVA and Tukey's multiple comparison tests (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Experiments were conducted twice with similar results and representative results from one experiment are given.