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. 2019 Nov 26;10:2749. doi: 10.3389/fimmu.2019.02749

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Copyright © 2019 Agallou, Athanasiou, Kammona, Tastsoglou, Hatzigeorgiou, Kiparissides and Karagouni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microarray time course analysis of DEGs. STEM analysis classified 629 DEGs of spleens from non-vaccinated (PBS) and vaccinated (p8-CPA_160−189) mouse groups at 0 (non-vaccinated non-infected), 4 and 16 weeks into 6 significant patterns of gene expression (p < 0.05). DEGs were classified into five main possible profiles according to the temporal gene expression pattern. Each square represents a pattern of gene cluster obtained by the STEM software. The number at the upper left corner of the box represents the profile ID number, and the black broken line in the box represents the overall trend in the expression of all genes in the gene set. The number in the lower left corner indicates the p-value assessing the similarity of the gene expression in the cluster.