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. 2019 Nov 26;10:2749. doi: 10.3389/fimmu.2019.02749

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Copyright © 2019 Agallou, Athanasiou, Kammona, Tastsoglou, Hatzigeorgiou, Kiparissides and Karagouni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR validation of the microarray data. (A) CXCL9, (B) CXCL11, (C) IL-21, (D) LAG3, (E) CD274, (F) PD-L2, (G) LILRB4, and (H) LGASL9 were validated. qPCR analysis for the expressions of the above genes was conducted in the RNA samples of non-vaccinated (PBS; black) and vaccinated (p8-CPA_160−189; red) mice at 4 and 16 weeks post challenge. The data are displayed as Log₂ fold-change relative to the expression in naïve (non-vaccinated non-infected) mice. Results are presented as means ± SD of three individual mice per group. (I) Correlation between microarray and qRT-PCR expression data for 9 selected genes at 4 and 16 weeks post challenge both in vaccinated and non-vaccinated mice. Correlation between microarray and qRT-PCR data was analyzed by Spearman's Rho test. The correlation coefficient was = 0.897, with a statistical significance of p < 0.001.