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. 2019 Nov 26;13:1256. doi: 10.3389/fnins.2019.01256

FIGURE 2.

FIGURE 2

Azithromycin elevates STAT3 phosphorylation in the motor cortex of rats subjected to 2 h MCAo followed by 2 h of reperfusion. (A) Representative immunofluorescence images of the contralateral and ipsilateral (ischemic) motor cortex (inset in B) of rats subjected to transient MCAo, showing expression of p-STAT3 in most GFAP-positive astrocytes and other DAPI-positive cells (likely resembling pyramidal neurons) in the lesioned hemisphere. Scale bars = 70 μm. (C) Western blot analysis of phospho-STAT3 (Tyr705) (p-STAT3), total STAT3, and tubulin performed on brain homogenates from the ipsilateral (Ipsi, dark bars) and contralateral (Contra, white bars) motor cortex. Rats were treated with vehicle (0.9% NaCl) or azithromycin (AZM, 150 mg/kg), i.p., upon reperfusion. Data from the densitometric analysis of the autoradiographic bands are expressed as mean ± SEM of four independent experiments (n = 4 rats per experimental group); ∗P < 0.05 vs. vehicle contralateral; #P < 0.05 vs. AZM contralateral and ∗∗P < 0.01 vs. vehicle ipsilateral (two-way ANOVA for repeated measures followed by Bonferroni post-test).