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. 2019 Nov 26;10:1376. doi: 10.3389/fpls.2019.01376

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Copyright © 2019 Zheng, Zhu, Wang, Hu, Xi, Xiao, Qu, Zhong, Fu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of kinetic parameters of UGT85A84 in linalool + UDP-Glc and linalool oxide + UDP-Glc reactions by the hyperbolic Michaelis-Menten saturation curve. (A) UDP-Glc (0.5mM) + linalool (5 µM to 50 mM). (B) UDP-Glc (0.5mM) + linalool oxide (5 µM to 5 mM). (C) linalool (0.5mM) + UDP-Glc (25 µM to 10 mM). (D) linalool oxide (0.5mM) + UDP-Glc (25 µM to 10 mM). The apparent Km and Vmax values for each sugar acceptor and the sugar donor were determined by Michaelis-Menten fitting in OriginPro 9.1 software.