Figure 3.

Comparative assessment of quality and yield for linear NA constructs using LNC and ANC methods. (A, B) Extension as a function of rotation for coilable 1.4 kb dsDNA (A) and 4 kbp dsRNA (B), as described in Figure 1B. Z-positions of the beads were measured at 0.3 pN (black) and at 4 pN (red) forces. Data at 0.3 pN were fitted with a Gaussian function (solid black line). (C) Fraction of coilable tethers obtained for DNA or RNA constructs assembled using either LNC or ANC strategy.