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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: Biopolymers. 2000;57(5):306–315. doi: 10.1002/1097-0282(2000)57:5<306::AID-BIP70>3.0.CO;2-7

Table I.

Multicomponent Analysis of DNA-Bound Donor Intensity Decays

1 Component 2 Components 3 Components
Acceptor Concn τi fi χR2 τi (ns) fi χR2 τi (ns) fi τ¯(ns) χR2
0 mM 2.33 1.00 36.9 2.53 0.81 1.64 2.28 0.68 2.47 1.53
(no acceptor) 0.51 0.19 0.31 0.18
3.34 0.14
2 μM 2.15 1.00 99.5 2.41 0.75 2.91 2.45 0.66 2.30 3.81
0.34 0.25 0.33 0.25
1.80 0.09
10 μM 1.69 1.00 374 2.23 0.55 4.50 2.34 0.94 2.05 2.47
0.35 0.45 0.003 0.04
0.67 0.02
20 μM 1.17 1.00 727 0.30 0.56 11.9 0.13 0.38 1.64 1.06
1.84 0.44 0.71 0.34
2.15 0.28
30 μM 0.75 1.00 1635 0.17 0.70 31.8 0.08 0.62 1.33 1.56
1.49 0.30 0.60 0.24
1.96 0.14
40 μM 0.57 1.00 1383 0.14 0.76 16.5 0.10 0.61 1.06 1.42
1.32 0.24 0.50 0.26
1.67 0.13
50 μM 0.34 1.00 1564 0.11 0.85 19.8 0.06 0.77 0.78 1.69
1.08 0.15 0.40 0.17
1.48 0.06

τ¯(ns) (ns), mean lifetime.