Figure 5.

Systemic inflammation in 5CreCAT mice. (A) Average spleen weight of 5Cre and 5CreCAT mice at 5.5 months of age. (B) Density of megakaryocytes in spleens of healthy and 5CreCAT mice, measured by vWF staining of tissue sections. (C,D) vWF-stained megakaryocytes in spleens of control 5Cre and 5CreCAT mice respectively; 100X. Insert in “D” is an enlarged image of a vWF stained megakaryocyte. (E) Gating of CD11b⁺ splenocytes for CX3CR1^lo Ly6C^hi inflammatory and CX3CR1^hi Ly6C^lo resident monocytes. (F) Densities of spleen infiltrating CD11b⁺ CX3CR1^lo Ly6C^hi inflammatory and CD11b⁺ CX3CR1^hi Ly6C^lo resident monocytes, measured by flow cytometry. (G) Frequencies of Ly6C⁺Ly6G⁺ myeloid derived suppressor cells in the spleen. (H) Treg to effector CD4⁺ T-cell ratio in the mesenteric lymph nodes of control 5Cre and of 5CreCAT mice. (I) RORγt⁺ Tregs relative to effector CD4⁺ T-cell in mesenteric lymph nodes, measured by flow cytometry. ^*p < 0.1; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001, unpaired Student's t-test. Age matched 5.5 months old 5Cre (n = 5) and 5CreCAT (n = 7) mice.