Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 3;10:2777. doi: 10.3389/fimmu.2019.02777

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Saadalla, Lima, Tsai, Osman, Singh, Linden, Dennis, Haeryfar, Gurish, Gounari and Khazaie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Transplant of 5CreCAT bone marrow in APC^Δ468 mice worsens colon polyposis and promotes expansion of CTMC like stromal mMCP5⁺ MCs. Mice were bone marrow reconstituted at 8 weeks of age and aged to 5.5 months, at which time they were analyzed. (A) Colon crypt lengths in C57BL/6 mice reconstituted with control 5Cre or 5CreCAT bone marrow. Measurements were from H&E stained sections at 200X magnification, using the line tool in ImageJ; each mark represents one crypt (B) Numbers of colon polyps in APC^Δ468 mice reconstituted with control 5Cre or 5CreCAT bone marrow; each mark represents one colon. (C) Densities of stromal or intraepithelial mMCP5 expressing MCs in the same polyps, counted in 100x fields; n = 4 mice per group. (D–O) Histology and immunohistology of 5 μm FFPE sections of adenomas. APC^Δ468 mice were lethally irradiated at 8 weeks of age and reconstituted with bone marrow from 5Cre or 5CreCAT mice, aged to 5.5 months and then analyzed; arrows point to adenomas. (D) Representative jelly roll of proximal small bowel of control 5Cre bone marrow chimeric APC^Δ468 mouse; H&E 16X. (E) Small bowel adenomatous polyp of the same; H&E 100X. (F) The same lesion; β-catenin stain 100X. (G) Representative jelly roll of proximal small bowel of 5CreCAT bone marrow chimeric APC^Δ468 mouse; H&E 16X. (H) Small bowel adenomatous polyp of the same; H&E 100X. (I) The same lesion, β-catenin stain 100X. (J) Representative colon jelly roll of control 5Cre bone marrow chimeric APC^Δ468 mouse; H&E 16X. (K) Colon adenomatous polyp of the same; H&E 100X. (L) The same lesion, β-catenin stain 100X. (M) Representative colon jelly roll of 5CreCAT bone marrow chimeric APC^Δ468 mouse; H&E 16X. (N) Colon adenomatous polyp of the same; H&E 100X. (O) The same lesion, β-catenin stain 100X. (P–R) Representative mMCP5 stained adenoma infiltrating mast cells. (P) Mast cells in the submucosal of 5Cre chimeric APC^Δ468 mouse colon adenoma. (Q,R) Mast cells in the submucosal and mucosal stroma respectively of 5CreCAT chimeric APC^Δ468 mouse colon adenoma. Significance in A-C was calculated by unpaired Student's t-test.