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. 2019 Nov 22;10:1132. doi: 10.3389/fgene.2019.01132

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Copyright © 2019 Ershova, Agafonova, Zakharova, Bravve, Jestkova, Golimbet, Lezheiko, Morozova, Martynov, Veiko, Umriukhin, Kostyuk, Kutsev, Veiko and Kostyuk

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative f-SatIII determination in the human DNA by NQH. (A) Photo of the membrane fragments with visualized f-SatIII. 50 ng of the denatured DNA samples (three spots for each DNA sample) from the HC-group (N = 31) and SZ-group (N = 30) were applied on the filter. 50 ng of six calibration DNA samples with a known amount of f-SatIII (shown in black rectangles) were also applied to the filter. For the background signal analysis 50 ng of a non-homologous to the DNA probe DNA sample was applied (in blue rectangles). (B) The filter was analyzed with the “Imager 6” soft. The average integral spot intensity and the standard error were determined. (C) The calibration dependence of the average signal intensity on the f-SatIII content in the calibration DNA samples. The equation of the curve that was used to calculate the f-SatIII content in the analyzed DNA samples is given. (D) The result of the quantitative f-SatIII determination in the DNA samples from HC and the SZ-group. For clarity, the data for each group are ranked according to the f-SatIII content.