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. 2019 Dec 10;20:959. doi: 10.1186/s12864-019-6314-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Label-free quantitative proteomic analysis of bumblebee queen haemolymph at six points within the life-cycle. Principal component analysis (PCA) of Z-score normalised label-free quantification (LFQ) intensity values for all statistically significant differentially abundant (SSDA) proteins (n = 79) identified (a) the first two components (PC1 and PC2) to explain 29.6 and 18.19% of the variance within the dataset, respectively. To further resolve differences in the haemolymph proteome, additional principal components (b) PC3, (c) PC4, and (d) PC5 were plotted individually against the first principal component. Each queen life stage is indicated by a unique colour