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. 2019 Dec 9;17(4):1559325819894148. doi: 10.1177/1559325819894148

Figure 8.

Figure 8.

A, Effect of linolenic acid (LA) and linoleic acid on endothelial nitric oxide synthase (eNOS) phosphorylation (at Ser1177) induced by acetylcholine (ACH) in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with ACH (10−5 M) alone for 4 minutes and pretreated with LA (5 × 10− 6 and 5 × 10− 5 M) and linoleic acid (5 × 10−5 M) for 1 hour, followed by ACH (10−5 M) for 4 minutes. Data (N = 3) are shown as mean ± standard deviation. N indicates the number of independent experiments. *P < .001 versus control. † P < .001 versus ACH alone. B, Effect of LA on eNOS phosphorylation (at Ser1177) induced by bupivacaine (BPV) in HUVECs. HUVECs were treated with BPV (3 × 10−4 M) alone for 10 minutes and pretreated with LA (5 × 10−5 M) for 1 hour, followed by BPV (3 × 10−4 M) for 10 minutes or LA (5 × 10−5 M) alone for 70 minutes. Data (N = 3) are shown as mean ± standard deviation. N indicates the number of independent experiments. *P < .01 and † P < .05 versus control. # P < .001 versus BPV alone. p-eNOS indicates phosphorylated endothelial nitric oxide synthase; t-eNOS: total endothelial NOS.