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. 2019 Dec 9;19:356. doi: 10.1186/s12906-019-2781-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Interference in the expression and distribution of T-bet in BM cells after 12 h of treatment. (a) Staining for T-bet (red) in BM cells was observed by confocal immunofluorescence microscopy. The stained cells (red) were quantitated with ImageJ. The scale bar corresponds to 60 μm throughout. (b) The quantified results are presented in a bar chart. a, Normal (N) group; b, Model (M) group; c and f, Radix Astragali (R) group; d and g, Radix Angelicae Sinensis (A) group; e and h, Radix Astragali + Radix Angelicae Sinensis (R + A) group; c, d and e were treated with 100 μg/mL of freeze-dried powders and f, g and h were treated with 250 μg/mL of freeze-dried powders. Data are presented as the mean ± SD, n = 3. *P < 0.05 and **P < 0.01