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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: Eur J Immunol. 2011 May 13;41(6):1742–1753. doi: 10.1002/eji.201041105

Figure 2.

Figure 2.

SHIP protein levels and activity decrease over time in response to IL-4 and correlate with an increase in M2 markers. SHIP+/+ BMMϕs were treated with IL-4 (10 ng/mL) for the indicated times. WCLs were analyzed for SHIP, SHIP2, PTEN, Ym1, argI, and Shc by Western blotting (A), SHIP immunoprecipitates were assayed for enzymatic activity (B), and 10 μg protein was used to determine arginase activity (C). SHIP+/+ BMMϕs were IL-4-treated followed by 24 h treatment with 10 ng/mL LPS, in the absence or presence of L-arginine. Clarified cell supernatants were analyzed for nitrite (D). Data in (A) are representative of three experiments and in (B–D) are the means±SEM of three independent experiments. *p<0.002 comparing control versus L-arginine supplementation (Student’s t-test).