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. Author manuscript; available in PMC: 2019 Dec 10.
Published in final edited form as: Eur J Immunol. 2011 May 13;41(6):1742–1753. doi: 10.1002/eji.201041105

Figure 6.

Figure 6.

Reducing SHIP protein levels increases argI expression and activity in response to IL-4 and enhances STAT6 transcription. (A) SHIP+/+ and SHIP+/− BMMϕs were either untreated (c) or treated with IL-4. WCLs were analyzed for SHIP, Ym1, argI, and GAPDH by Western blotting. Data shown are representative of three experiments with similar results. (B) RAW264.7 cells were treated with SHIPsiRNA or nsRNA for 3 days. WCLs were analyzed for SHIP and GAPDH by Western blotting. Densitometry values are relative to GAPDH and normalized to nsRNA control. Data shown are representative of four experiments with similar results. (C) RAW264.7 cells were treated with SHIPsiRNA or nsRNA for 6h and were treated or not with IL-4. WCLs were collected and 10 μg of protein was assayed for arginase activity. (D) RAW264.7 cells were transfected with pSTAT6-luciferase in the presence of SHIP siRNA or nsRNA. After 48h, cells were stimulated ±IL-4 for 24h. WCLs were analyzed for luciferase activity. Data in (C, D) are the means±SEM of three independent experiments assayed in duplicate. ns, not significant; *p<0.05 comparing SHIP siRNA with nsRNA (Student’s t-test).