Fig. 2.

Analysis of accumulation of recombinant GFP in N. benthamiana. a Representative TMV vector-infiltrated N. benthamiana leaves under UV-illumination at 8 days post-inoculation (dpi). b Confocal microscopy image of GFP in infiltrated leaves. Green signal in image is due to GFP fluorescence. c Coomassie-stained polyacrylamide gel showing GFP accumulation in infiltrated N. benthamiana leaves. M, protein molecular weight marker in kDa; H, total soluble protein extracts from non-inoculated leaves, negative control; lane 4, 6 and 8, extracts from infiltrated leaves at 4, 6 and 8 dpi, respectively. d Western blot analysis of GFP from infiltrated N. benthamiana leaves. M, protein molecular weight marker in kDa; H, total soluble protein extracts from non-inoculated leaves, negative control; lane 4, 6 and 8, extracts from infiltrated leaves at 4, 6 and 8 dpi, respectively