Table 1.
Processing step | TSPa (mg) | GFPb (mg) | Purityc (%) | GFP Yield d (%) |
---|---|---|---|---|
Extract | 13.7 | 8.2 | 59.9 | 100.0 |
Ethanol extraction | 5.5 | 4.8 | 87.3 | 58.5 |
n-butanol extraction | 4.7 | 4.2 | 89.4 | 51.2 |
HiScreen Capto Butyl | 2.9 | 2.8 | 96.6 | 34.1 |
aAmount of the TSP was determined according to BCA
bAmount of the GFP was measured by the ELISA
cPurity is defined as the amount of GFP divided by the amount of TSP in the same sample
dGFP Yield is defined as the amount of GFP recovered divided by initial amount of GFP in the crude extract of inoculated leaves