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. 2019 Dec 9;19:86. doi: 10.1186/s12896-019-0590-y

Table 1.

Quantitative specifications of the principal stages of GFP purification

Processing step TSPa (mg) GFPb (mg) Purityc (%) GFP Yield d (%)
Extract 13.7 8.2 59.9 100.0
Ethanol extraction 5.5 4.8 87.3 58.5
n-butanol extraction 4.7 4.2 89.4 51.2
HiScreen Capto Butyl 2.9 2.8 96.6 34.1

aAmount of the TSP was determined according to BCA

bAmount of the GFP was measured by the ELISA

cPurity is defined as the amount of GFP divided by the amount of TSP in the same sample

dGFP Yield is defined as the amount of GFP recovered divided by initial amount of GFP in the crude extract of inoculated leaves