Table 3.

Estimation of k_cat/K_m and product profile of the processive cellulases on cello-oligosaccharides with DP 5–8

Enzyme name	kcat/Km (min−1 M−1)				Mode of actiona
	C5	C6	C7	C8	C5	C6	C7	C8
1. CcCel48A	1.37 × 103	2.89 × 103	4.35 × 103	4.41 × 103	C3 + C2	C4 to C2	C4 to C1	C5 to C1
2. CtCbh5A	8.18 × 103	8.15 × 103	8.3 × 103	9.01 × 103	C3 + C2	C4 to C2	C4 to C2	C6 to C1
3. CtCel9A	6.45 × 102	6.76 × 102	2.55 × 102	9.3 × 101	C3 + C2	C4 to C2	C4 to C2	C5 to C2
4. CtCbh9A	7.46 × 102	7.61 × 102	2.01 × 102	2.36 × 102	C4 to C1	C4 to C2	C4 to C2	C5 to C2
5. CtCel9B	1.71 × 103	1.56 × 103	1.59 × 103	1.88 × 103	C3 + C2	C4 to C2	C5 to C2	C6 to C2
6. PaCbh6A	5.13 × 103	3.46 × 104	8.87 × 104	8.11 × 104	C3 + C2	C4 to C2	C4 to C2	C5 to C2
7. CcCel9W	1.13 × 103	8.74 × 103	2.18 × 104	2.86 × 104	C3 + C2	C4 to C2	C4 to C2	C5 to C2
8. CcCel9M	2.99 × 104	2.52 × 104	3.17 × 104	2.78 × 104	C4 + C1	C4 + C2	C4 to C2	C5 to C2
9. CcCel9R	–	2.04 × 103	1.23 × 103	8.51 × 102	–	C3	C4 + C3	C5 to C3
10. CcCel9A	1.37 × 103	1.34 × 103	1.34 × 103	1.79 × 103	C4 to C1	C4 to C2	C5 to C2	C4 to C2
11. CsCbh48A	1.00 × 103	1.56 × 103	2.97 × 103	3.70 × 103	C3 + C2	C4 to C2	C6 to C1	C6 to C1
12. CcCel9J	–	–	–	–	–	–	–	–
13. CcCel9Q	3.26 × 103	3.81 × 103	5.04 × 103	6.51 × 103	C3 + C2	C4 to C2	C4 to C2	C6 to C2
14. RfCel9A	1.12 × 102	1.94 × 103	5.77 × 103	5.93 × 103	–	C4 to C2	C5 to C2	C6 to C2

^a“C2 + C3” represents the products C2 and C3, while “C4 to C1” represents a range of products with degree of polymerization varying between 4 and 1, i.e., from cellotetraose to glucose (C4 + C3 + C2 + C1)