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. 2019 Dec 9;12:135. doi: 10.1186/s13045-019-0830-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The reduction of YAP m⁶A modification inhibits tumor growth and enhances DDP sensitivity in vivo. a The expressions of METTL3, MALAT1, YTHDF3 and YAP were analyzed by RT-PCR (left panel) and qPCR (right panel) in the A549 cells with transfections of control^vector, METTL3^Vector, METTL3^shMALAT1, METTL3^shYTHDF3 or METTL3^shYap, respectively. b, c Xenografted A549 cell tumors with stably expressing control^vector, METTL3^Vector, METTL3^shMALAT1, METTL3^shYTHDF3 and METTL3^shYap in mice and the dimensions were measured at regular intervals. d Representative H&E-stained microscopic images of the mouse lung tumors originated from xenografted A549 cells with stably expressing control^vector, METTL3^Vector, METTL3^shMALAT1, METTL3^shYTHDF3 and METTL3^shYap by subcutaneous injection. e, f The protein expression levels of Ki67, YAP, vimentin, E-cadherin, ABCG2 and ERCC1 were analyzed by immunohistochemical staining (n = 5) (e), western blot and RT-PCR (f) in the xenografted A549 cell tumors with stably expressing control^vector, METTL3^Vector, METTL3^shMALAT1, METTL3^shYTHDF3 and METTL3^shYap of mice. g Kaplan–Meier overall survival (OS) curves of the mice with transfected of A549 cells with stably expressing control^vector, METTL3^Vector, METTL3^shMALAT1, METTL3^shYTHDF3 and METTL3^shYap. h, i Xenografted A549 cell tumors with stably expressing control^vector, METTL3 ^Vector and METTL3^shYap were treated with PBS or DDP and the dimensions were measured at regular intervals. j, k The tumor nodules from control^vector, METTL3 ^Vector and METTL3^shYap groups were treated with PBS or DDP every three days. The protein expression levels of Ki67, ABCG2 and ERCC1 were analyzed by western blot, RT-PCR j and immunohistochemical staining assays (n = 5) (k). l Kaplan–Meier overall survival (OS) curves of the mice that were transfected of A549 cells with stably expressing control^vector, METTL3^Vector and METTL3^shYAP by treatment of DDP or PBS every three days. Results were presented as mean ± SD three independent experiments. **P < 0.01 indicates a significant difference between the indicated groups. NS, not significant