Fig. 4.

Overexpressed lncRNA PAXIP1-AS1 affects glioma cell migration, invasion, and angiogenesis by recruiting transcription factor ETS1 to upregulate KIF14 expression. a, Transwell assay to examine the migration ability of glioma cells (200 ×). b, Transwell assay to examine the invasive ability of glioma cells (200 ×). c, The tube formation assay to examine angiogenesis of HUVECs cocultured with TJ905 cells (100 ×). d, Western blot analysis for expression of E-Cadherin, N-Cadherin, MMP-2, VEGF-A, ETS1 and KIF14 normalized to GAPDH in glioma cells. * p < 0.05 vs. the oe-NC + sh-NC group, # p < 0.05 vs. the oe-PAXIP1-AS1 + oe-ETS1 + sh-NC group. The values are measurement data and expressed as mean ± standard deviation. Comparisons among multiple groups were made by one-way ANOVA with Tukey’s post hoc test. Experiments were repeated three times independently