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GH induces the phosphorylation of STAT5 but not AMPK in the hypothalamus. A–C) Western blotting of hypothalamic samples collected from wild-type C57BL/6 mice that received either saline (n = 5) or GH injection (i.p.; n = 4 each time) 15, 30, 45, 60, and 90 min prior to tissue collection. The phosphorylation of STAT5-Tyr694 (B), AMPK-Thr172 (C), and GAPDH (for normalization) was determined. Values are means ± sem. *P < 0.05 vs. saline injection (Bonferroni’s multiple comparisons test).
