Figure 7.

Suppression of TREM2 in microglia is detrimental to cultured neurons. The control and TREM2-knockdown BV2 cells were treated with α-syn for 4 h. CM was then harvested and added into SH-SY5Y neuron culture for another 24 h. A, B) The apoptosis of SH-SY5Y cells were assessed by Annexin V-PI staining using flow cytometry (A), and the apoptotic rates were shown in graph (B). C) The cell viability of SH-SY5Y cells were detected using MTT assay. Data are presented as the mean ± sem of triplicate independent experiments. *P < 0.05, **P < 0.01.