Figure 7.

Protein synthesis in tenocytes treated with IGF1. Representative Western blot for proteins that have incorporated puromycin (A) and quantification of band densitometry for tenocytes (B) that were untreated or were treated with IGF1, IGF1 and cycloheximide, IGF1 and wortmannin, or IGF1 and PD98059. C) Representative Western blots for p-ERK1/2^T202/Y204, total ERK1/2, p-ELK^S383, total ELK, IRS1^Y608, IRS1^S612, total IRS1, p-Akt^T308, and total Akt in untreated cells or in cells treated with IGF1, IGF1 and wortmannin, or IGF1 and PD98059. Differences tested with a 1-way ANOVA: significantly different (P < 0.05) from −IGF1 controls. Coomassie stained membranes are shown as total protein loading controls. Values are means ± CV; n ≥ 4 replicates/group.