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. 2019 Aug 26;33(11):12630–12643. doi: 10.1096/fj.201901087RR

Figure 5.

Figure 5

Transcriptional regulation of KLF5 by HIF-1 plays a key role in TIF. A) Hierarchical clustering shows distinct mRNA expression profiling among groups. The red and green shades indicate the expression above and below the relative expression, respectively, across all samples. B) KLF5 protein expression in the kidney cortex after MK treatment. **P < 0.01 vs. 5/6Nx-V, #P < 0.01 vs. 5/6Nx-5 mpk. (Means ± sem; n = 6–9). C) Representative data of KLF5 immunostaining in kidney. Scale bars, 100 μm. Representative immunofluorescence double staining of AQP1 (green, proximal tubules), NCC (green, distal tubules), AQP2 (green, collecting ducts), and KLF5 (red) in kidneys of mice. Scale bars, 40 μm. D) Chromatin immunoprecipitation assays show binding of HIF-1 to KLF5 promoter, which is mediated by MK stimulation (1000 nM for 18 h). E) Effect of KLF5 knockdown using KLF5 siRNA on protein expression of α-SMA, collagen I, and fibronectin in HK-2 cells treated with MK for 24 h, as determined by Western blotting. ADPRHL1, ADP-ribosylhydrolase-like 1; ATF3, activating transcription factor 3; DOK3, docking protein 3; FGFR1, fibroblast growth factor receptor 1; FOXC1, Forkhead box C1; GAD1, glutamate decarboxylase 1; GALNT18, polypeptide N-acetylgalactosaminyltransferase 18; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL1RAP, interleukin 1 receptor accessory protein; IL6R, interleukin 6 receptor; ISM2, isthmin 2; ITPR1, inositol 1,4,5-trisphosphate receptor type 1; RASD2, RASD family member 2; RHBDL1, rhomboid-like 1; SIRT2, sirtuin 2; TGFA, TGFα; TNFAIP3, TNFα-induced protein 3; SMAD9, SMAD family member 9; TPSG1, tryptase γ1. **P < 0.01 vs. vehicle (means ± sem; n = 3).