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. 2019 Sep 18;33(11):12960–12971. doi: 10.1096/fj.201900932R

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© The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 2.0 International (CC BY-NC-ND 2.0) (https://creativecommons.org/licenses/by-nc-nd/2.0/) which permits noncommercial use, distribution, and reproduction in any medium, but prohibits the publication/distribution of derivative works, provided the original work is properly cited.

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Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, FGFR3, and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.