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. 2019 Sep 18;33(11):12960–12971. doi: 10.1096/fj.201900932R

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© The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 2.0 International (CC BY-NC-ND 2.0) (https://creativecommons.org/licenses/by-nc-nd/2.0/) which permits noncommercial use, distribution, and reproduction in any medium, but prohibits the publication/distribution of derivative works, provided the original work is properly cited.

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Analysis of PTK7 and FGFR1 binding. A) Western blot analysis following reciprocal coimmunoprecipitation of FGFR1-HA with full-length PTK7 (PTK7-FLAG), PTK7 extracellular and TM domains (PTK7-Ext-TM-FLAG), or PTK7 TM and Cyt domains (PTK7-TM-Cyt-FLAG) in HEK293 cells. An empty vector served as the negative control. GAPDH served as a loading control for the total cell lysate. B) Western blot analysis following immunoprecipitation of FGFR1-HA with the purified PTK7 extracellular domain (sPTK7-His) in HEK293 cells (top). sPTK7-His was pulled down with Ni²⁺-NTA agarose (bottom). An empty vector served as the negative control. GAPDH served as the loading control for total cell lysates.