Figure 4.

Colocalization of PTK7 and FGFR1 in HEK293 cells. Serum-depleted HEK293 cells transiently expressing PTK7-FLAG and FGFR1-HA were fixed, permeabilized, and stained with mouse anti-FLAG, rabbit anti-HA antibodies, Alexa Fluor Rhodamine Red-conjugated goat anti-mouse IgG, Alexa Fluor 488–conjugated goat anti-rabbit IgG antibodies, and DAPI. The fluorescence signals were analyzed by confocal fluorescence microscopy. The confocal images on the upper and lower panels were taken at the bottom and in the middle of the cells, respectively. Scale bars, 10 μm.