TABLE 1.

Primer sequences for real-time RT-PCR analysis of RPTKs and PTK7

Gene	Nucleotide sequence, 5′–3′		Nucleotide positiona		GenBank accession no.
	Forward	Reverse	Forward	Reverse
FGFR1	CCTGGTGACAGAGGACAAGTGATG	CCGGTCAAATAATGCCTCGGG	2829–2853	2967–2946	NM_023110.2
FGFR2	CCCCCTGCGGAGACAGGTAAC	CAGGGGCTTGCCAGTGTC	1913–1934	2108–2090	NM_000141.4
FGFR3	CCGTGACGTCCACCGACGAG	GGGGACCAGTGGCCCTTCAC	2514–2534	2693–2673	NM_000142.4
FGFR4	GCGGCGTCCACCACATTGAC	CACCCGGTCAAACAAGGCCTC	2119–2139	2210–2189	NM_002011.4
FGFRL1	AGGGCAAGGTCCACCAGCAC	TGCTGGCCATTCCTCCTCGC	1627–1647	1780–1760	NM_001004356.2
FLT1	CCTCCTGCGAAACCTCAGTG	AGGCTCGGGGACACCATTAG	2271–2290	2355–2336	NM_002019.4
KDR	TTGGTGGAATGGTGCCCAGC	AGTGTGGTCCCCGAGTCAGGC	4147–4167	4354–4333	NM_002253.2
FLT3	GTTCACGAACCCCCTGAAATTG	ACACGTGCTCTGGAAGGAATG	3556–3577	3618–3598	NM_004119.2
FLT4	GCACAGGAGTGTCCTCTTCC	TCTGAATCTCAGGGGGAGGG	4274–4293	4415–4396	NM_182925.4
c-KIT	AGGAGTGGGAAAACACTGCC	AGGACCCTCTTGGACTCAGG	4010–4029	4194–4175	NM_000222.2
PDGFRB	GTCCTTCAGGCCCATCAGTC	CCGTCTGGCCTTCTAGACTC	4507–4526	4590–4571	NM_002609.3
CSF1R	CGTCTGGTCCTATGGCATCC	GGGTAGGGATTCAGCCCAAG	2803–2822	2862–2843	NM_005211.3
PTK7	AGAGATGCCCCATGGTGGGC	ACGGCTTGCTGTCCACGGTG	3156–3176	3359–3339	U40271
GAPDH	ACTGCTTAGCACCCCTGGCCA	TTGGCAGTGGGGACACGGAAG	488–508	740–720	BC023632

^aNucleotide position of cDNA target sequence is shown.