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AKO, BKO, and DKO in ACH-3P cells and its effect on let-7 miRNAs. A–C) Representative immunoblots for LIN28A, LIN28B, and α-tubulin and densitometric analysis of LIN28A and LIN28B in AKOs, BKOs, and DKOs compared with SC (n = 3). D) Comparison of let-7 miRNAs in SC vs. AKO vs. BKO vs. DKO (n = 3). Letters (a–d) indicate a significant difference (P < 0.05) in let-7 miRNA by treatment. *P < 0.05 vs. SC.
