Figure 3.

The PHD2-HIF-α axis in neurons controls vessel growth in the murine CNS during early postnatal development. The density of vessel segments (A), endothelial cells (B), and vascular branching points (C) was determined by CD31 immunofluorescence staining in brain sections from 0 to 1-wk-old Phd2^f/f, nPhd2^Δ/Δ, Hif1a/Hif2a^ff/ff, nHif1a/Hif2a^ΔΔ/ΔΔ, Phd2/Hif1a/Hif2a^fff/fff, and nPhd2/Hif1a/Hif2a^{ΔΔΔ/ΔΔΔ} mice (means ± sd; n = 3/group; 2-way ANOVA with Holm-Sidak’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001.