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. 2019 Aug 26;33(11):12336–12347. doi: 10.1096/fj.201900693R

Figure 3.

Figure 3

PKM2 is oxidized in FH-deficient cells. A, B) UOK262 (A) and UOK268 (B) (FH-WT and FH-deficient) cells were processed for ATSP. WCL and ATS-bound proteins were analyzed by IB with the indicated antibodies. The relative amounts of PKM2 and TKT oxidation are quantified below. Data represent means ± sem (n = 6), unpaired, 2-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. C) UOK262 (FH-WT and FH-deficient) cells were processed for ATSP or ATSP-total. WCL and ATS-bound proteins were analyzed by IB with the indicated antibodies. HIF1α, hypoxia-inducible factor 1 α; PD, pull down; PGK, phosphoglycerate kinase; PGM, phosphoglycerate mutase.