Figure 4.

Adhesion and internalization are required for TEV–mediated B-cell IgG secretion. Primary splenic B cells (1 × 10⁷) pretreated with RGD peptide and/or Dynasore were treated with TEVs, and cells and cell lysates were used to evaluate EV-mediated signaling. A, B) ImageStream flow cytometry and flow cytometry were performed to evaluate the fluorescence intensity of TEVs internalized into cells. C) IgG levels in the B-cell culture supernatants at 72 h. D) Western blot analysis of B-cell protein levels. E) qPCR analysis of B-cell gene expression at 24 h. F) qPCR analysis of B cell gene expression at 72 h; n = 3–5, data are presented as means ± sd. *P < 0.05 vs. the PKM2^fl/fl-TEV group (1-way ANOVA followed by Tukey’s test for multiple comparisons).