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. 2019 Sep 7;33(11):12873–12887. doi: 10.1096/fj.201900107R

Figure 7.

Figure 7

Gal-1 enhances the attachment of Syn-2–pseudotyped viruses to cells. A) Schematic representation of the experiment. B) HEK293T cells (triplicate samples) were infected by Syn-2–pseudotyped viruses at 4°C in the presence or absence of PBS (virus only), 4 µM Gal-1 (light gray bars), or 4 µM Gal-1 + lactose (Lact; dark gray bars) (Gal-1 during spinoculation). After spinoculation, cells infected without Gal-1 were incubated for 1 h at 37°C with either PBS, 4 µM Gal-1, or 4 µM Gal-1 + lactose (Gal-1 after spinoculation). At 24 h postinfection, normalized luciferase activities were calculated and are represented as the mean normalized luciferase activity ± sem of 3 independent experiments. Ns, not significant ***P < 0.001 (2-way ANOVA).