Figure 10.

A–C) Deletion of P-selectin exacerbates HIF1-α abundance in diet-induced obesity. Compared to WT mice fed a low fat diet (A), mice given an HFD for 10 consecutive weeks experienced up-regulation of HIF1-α (B), which was further exacerbated by deletion of P-selectin (C) (arrows indicate positive brown peroxidase reaction for HIF1-α staining). D) Bar graph shows quantification of data from all experimental groups of mice. Values are means ± sem. Numbers at the bottom of bars are the number of mice studied in each group. In total, 2–5 tissue sections were studied in the VAT isolated from each mouse. LFD, low-fat diet; P-sel, P-selectin.