Figure 3.

Genetic deletion of P-selectin abolishes LEIs induced by HFM. With administration of single HFMs (A, B) and 24-h HFD (C, D), WT mice experience a time-dependent increase in leukocyte rolling and adhesion in the VAT microcirculation of the mesentery. P-selectin–deficient mice are protected from inflammatory activation of the VAT microcirculation. Time-course studies also demonstrate that the inflammatory response to a single HFM peaks at 2 h and subsides at 4 h. Data are means ± sem. Numbers at the bottom of bars indicate the number of mice studied in each group. BSLN, baseline; LFD, low-fat diet; LFM, low-fat meal; Psel, P-selectin.