Figure 4.

Neutrophils accumulate in the VAT following acute fat overload. Epididymal VAT samples were collected before high-fat feeding [baseline (BSLN)] and at 2 and 4 h following a single HFM, as well as after 24 h of ad libitum HFD feeding. SVFs were isolated and stained with antibodies for neutrophils [Ly6G (A)] lymphocytes [CD4 (B)] and monocytes [F480 (C)]. Neutrophils were found to be the main leukocyte subpopulation to postprandially infiltrate VAT following consumption of HFMs. Values are means ± sem from 3 mice in each experimental group. Three samples were obtained from each mouse. NS, not significant; P-sel, P-selectin.