Figure 6.

Palmitate causes cell surface expression of P-selectin in HCAECs. A) Representative photomicrographs illustrating the effect of palmitate on P-selectin cell surface expression are shown. Green fluorescence emission indicates positive staining. Histamine was used as a positive control because of its established ability of up-regulating P-selectin. B) Bar graphs show densitometry quantification of P-selectin staining in HCAECs. Values are means ± se. Three independent experiments were performed for each quantification (indicated by numbers at the bottom of bars). Images were obtained at ×40 magnification.