Figure 9.

Deletion of P-selectin fails to attenuate TNF-α up-regulation in the VAT while preventing TNF-α in the plasma. WT mice given an HFD for 10 consecutive weeks experienced elevated levels of TNF-α in the epidydimal fat, which were not prevented by deletion of P-selectin [photomicrographs and bar graph (A); arrows indicate positive brown peroxidase reaction for TNF-α staining]. With longer 16-wk feeding, TNF-α levels increased in the plasma of WT mice but not in that of P-selectin–deficient mice [bar graph (B)]. Values are means ± sem. Numbers at the bottom of bars are the number of mice studied in each group. In total, 2–5 tissue sections were studied in the VAT isolated from each mouse. LFD, low-fat diet; Psel, P-selectin