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. 2019 Sep 7;33(11):12972–12982. doi: 10.1096/fj.201901145RR

Figure 4.

Figure 4

Effects of PNE on the expression of the main components of the local bone RAS in the male offspring. A) qRT-PCR analysis of the gene expression of RAS, including ACE and angiotensin receptors, and ELISA analysis of Ang II production in the bone tissue from GD20 to PW12. B) Representative immunostaining images of ACE in the bone tissue from GD20 to PW12. C) Quantitative immunostaining analysis of the mean optical density of ACE from GD20 to PW12; n = 8/group. Data are expressed as means ± sem. *P < 0.05, **P < 0.01 compared with the controls.