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. 2019 Sep 7;33(11):12972–12982. doi: 10.1096/fj.201901145RR

Figure 5.

Figure 5

Effects of PNE on the histone acetylation level of ACE and its intrauterine programming mechanism in the male offspring. A) ChIP assay of H3K9ac, H3K14ac, and H3K27ac levels in the ACE promoter region on GD20. B) ChIP assay of the H3K9ac, H3K14ac, and H3K27ac levels in the ACE promoter region at PW2. C) ChIP assay of the H3K9ac, H3K14ac, and H3K27ac levels in the ACE promoter region at PW12. D) qRT-PCR analysis of the nicotine receptor expression in the fetal and adult bone tissue from the control and PNE groups. E) qRT-PCR analysis of the gene expression of p300 in the fetal and adult bone tissue from the control and PNE groups; n = 8/group. Data are expressed as means ± sem. *P < 0.05, **P < 0.01 compared with the controls.