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. 2019 Sep 7;33(11):12972–12982. doi: 10.1096/fj.201901145RR

Figure 7.

Figure 7

The molecular mechanism of the activated RAS induced by nicotine in the BMSCs. A) qRT-PCR analysis of the gene expression of RAS, including ACE, angiotensin receptors, and ELISA analysis of Ang II production in the BMSCs cotreated with nicotine and DH-β-E. B) ChIP assay of H3K9ac levels in the ACE promoter region in the BMSCs treated with nicotine or cotreated with nicotine and DH-β-E. C) Western blotting assay of the p300 protein levels in the nucleus after treating the BMSCs with nicotine or cotreating the BMSCs with nicotine and DH-β-E. D) ChIP assay of the H3K9ac levels in the ACE promoter region after cotreating the BMSCs with nicotine and p300 inhibitor C646. All of the experiments were conducted at least 3 times. Data are expressed as means ± sem. *P < 0 0.05, **P < 0.01 compared with the untreated cells.