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. 2019 Aug 7;33(11):12008–12018. doi: 10.1096/fj.201802702R

Figure 7.

Figure 7

Let-7–dependent mechanism of Lin28A overexpression. A) qRT-PCR analysis of mature Let-7a and Let-7g in embryonic lungs derived from E14.5, E16.5, and E18.5 embryos. Asterisks indicate comparisons between different embryonic days, and pluses indicate comparisons between transgene and control samples. */+P < 0.05, **/++P < 0.0001; n = 3. B) Five littermates, 2 d old, from the “rescue” experiment. i7s, mice overexpressing only i7s; Lin28A;i7s, mice overexpressing both Lin28A and i7s. Two additional embryos were found dead after delivery; therefore, they do not appear in the figure. As expected, these mice inherited only the Lin28A overexpression cassette and not the i7s isoform. C) qRT-PCR analysis of the 7 mice described above. Left panel, Lin28A expression levels. Right panel, mature Let7s levels. Note the high Let-7g levels in the Lin28;i7s mouse. The 2 last samples in each panel (“Lin28a”) are from the dead embryos that overexpressed only Lin28A and not i7s. D) Top 10 misregulated pathways in the E14.5 transgenic lungs, identified by IPA software.