Figure 3.

Intracellular signal transduction of BMP9 and BMP2 in mouse primary osteoblasts. A) After serum starvation for 4 h, primary osteoblasts derived from newborn mouse calvariae were stimulated with BMP2 or BMP9 (50 ng/ml) for the indicated minutes. Cell lysates were analyzed with the indicated antibodies by Western blotting. B) Phosphorylation levels of GSK3-β and ERK, as well as the protein expression level of β-catenin, were photographically measured as in Fig. 2B. Fold inductions of phosphorylation levels and β-catenin protein expression for BMP9 and BMP2 stimulation were calculated and shown as graphs as in Fig. 2B. P, phosphorylated.