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. 2019 Sep 26;33(11):12915–12928. doi: 10.1096/fj.201900803R

Figure 1.

Figure 1

SMAD5-AS1 is highly expressed in NPC tissues and cells. A) The heatmap of NPC-related gene expression dataset GSE64634. B, C) Expression of SMAD5-AS1 in the NPC tissues (n = 50) and the normal nasopharyngeal epithelial tissues (n = 30) determined by RNA in situ hybridization assay. D) The expression of SMAD5-AS1 in the NPC tissues and the normal nasopharyngeal epithelial tissues determined by qRT-PCR. E) The expression of SMAD5-AS1 in the normal cell line and the NPC cell lines determined by qRT-PCR. Measurement data were expressed as means ± sd. Comparisons between NPC tissues and normal nasopharyngeal epithelial tissues were analyzed via Student’s t test and the comparisons among multiple groups were analyzed by 1-way ANOVA, followed by Tukey’s’s post hoc test. The experiment was repeated 3 times. *P < 0.05 vs. the normal nasopharyngeal epithelial tissues; #P < 0.05 vs. the NP69 cell line; &P < 0.05 vs. the CNE1 cell line ZNF197, zinc finger protein 197; NEURL4, neuralized homologue 4; AKR1D1, aldo-keto reductase 1D1; SMAD5-AS1, SMAD5 antisense RNA 1; NME5, non-metastatic cells 5; RAB17, ras-related protein 17; PRKAA2, activated protein kinase-alpha2.