Figure 2.

SMAD5-AS1 silencing inhibits cell proliferation, invasion, migration, and EMT in NPC. A) The expression of SMAD5-AS1, E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin in cells treated with pc-DNA SMAD5-AS1 determined by qRT-PCR. B) Gray value analysis of E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin in cells treated with pc-DNA SMAD5-AS1. C) Protein expression of E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin detected by Western blot analysis in cells treated with pc-DNA SMAD5-AS1. D, E) Cell proliferation of cells treated with pc-DNA SMAD5-AS1 measured by EdU assay. F, G) Cell invasion of cells treated with pc-DNA SMAD5-AS1 evaluated by Transwell assay. H, I) Cell migration of cells treated with pc-DNA SMAD5-AS1 measured by scratch test. J) The expression of SMAD5-AS1, E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin in cells treated with empty vector, si-SMAD5-AS1-1, and si-SMAD5-AS1-2 determined by qRT-PCR. K) Gray value analysis of E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin in cells treated with empty vector NC, si-SMAD5-AS1-1, and si-SMAD5-AS1-2. L) Protein expression of E-cadherin, N-cadherin, MMP-9, Snail, and Vimentin in cells treated with empty vector, si-SMAD5-AS1-1, and si-SMAD5-AS1-2 detected by Western blot analysis. M, N) Cell proliferation of cells treated with empty vector, si-SMAD5-AS1-1, and si-SMAD5-AS1-2 estimated by EdU assay. O, P) Cell invasion of cells treated with empty vector, si-SMAD5-AS1-1, and si-SMAD5-AS1-2 measured by Transwell assay. Q, R) Cell migration of cells treated with empty vector, si-SMAD5-AS1-1, and si-SMAD5-AS1-2 detected by scratch test. Measurement data were expressed as means ± SD. Comparison between 2 groups was analyzed by nonpaired Student's t test and comparisons among multiple groups were analyzed by 1-way ANOVA, followed by Tukey's post hoc test. The experiment was repeated 3 times. *P < 0.05 vs. the vector group; #P < 0.05 vs. the NC group.