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PMCA products from M83 mouse brain contain partially PK-resistant α-syn species. Immunoblots of α-syn (C-20R antibody) in 10% brain homogenates from M83+/−, D, and C57Bl/6-WT mice treated or not by 144 PMCA cycles after PK digestion for 30 min at 0-, 0.5-, 1-, 2.5-, 5-, and 10-μg/ml final concentrations. Bars to the left of each panel indicate the ∼20-, 30-, and 40-kDa MW markers. M, MW marker; Rec, human recombinant α-syn control; WT, wild type.
