TABLE 1.

α-Syn PMCA seeding activities in a panel of brain samples from sick or healthy M83 mice

Reference	Inoculation	Status	Age at death (day)	α-Syn aggregate detection by Western blot
				M83+/− substrate (1 round)	C57Bl/6-WT substrate (round 1/2)
1	—	Healthy	60	−	−/−
2	—	Healthy	90	−	−/−
3	—	Healthy	90	−	−/−
4	—	Healthy	90	−	−/−
5	—	Healthy	110	−	−/+
6	—	Healthy	110	−	−/+
7	—	Healthy	110	−	−/+
8	—	Healthy	146	−	−/+
9	—	Healthy	146	−	−/+
10	—	Healthy	146	−	−/−
11	—	Healthy	146	−	−/+
12	—	Healthy	180	−	−/−
13	—	Healthy	180	−	−/−
14	Fibrils	Sick	139	+	+/+
15	Fibrils	Sick	139	+	+/+
16	Sick M83 brain (1%)	Sick	141	+	+/+
17	Sick M83 brain (5%)	Sick	142	+	−/+
18	Sick M83 brain (5%)	Sick	161	+	−/+
19	Fibrils	Sick	182	+	+/+
20	—	Sick	365	+	+/+
21	—	Sick	451	+	+/+
22	—	Sick	457	+	+/+

One or 2 PMCA rounds were performed in M83^+/− (5%, 96 cycles) or C57Bl/6-WT (10%, 144 cycles) brain substrate after seeding with whole-brain homogenates of M83^+/+ mice diluted at 10⁻⁴ or 10⁻³ for sick or healthy mice, respectively. The sick M83^+/+ mice were either old unchallenged mice (≥1 yr old) or 3–6-mo-old mice after intracerebral inoculations of brain extracts of sick M83^+/+ mice or of recombinant fibrils. The Western blot detection results represent the presence (+) or absence (−) of SDS-resistant α-syn aggregates detected with C-20R antibody, as shown in Fig. 6.