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. 2019 Jul 30;33(11):11706–11720. doi: 10.1096/fj.201900698R

Figure 1.

Figure 1

Phosphorylation levels of SHP2 and ROCK in eosinophils from BALFs of children with allergic asthma or FBA and of mice sensitized with OVA and challenged with OVA or NS. A, B) BALF cells from children with FBA or asthma were used for immunostaining of CCR3 and p-Y542-SHP2 and p-S1366-ROCK2 or isotype IgG of p-SHP2 (Y542), p-S1366-ROCK2, and DAPI, and subsequent quantification of p-Y542-SHP2 or p-S1366-ROCK2 in CCR3-positive cells. C, D) OVA-sensitized mice were aerosolized with 1% OVA or an equal volume of NS for 30 min once daily for 7 d. Twenty-four hours after the last OVA challenge, BALF cells were prepared for the immunostaining of CCR3, p-Y542-SHP2, p-S1366-ROCK2, and DAPI, and subsequent quantification of p-Y542-SHP2 or p-S1366-ROCK2 in CCR3-positive cells. White arrows indicate the positive p-Y542-SHP2 or p-S1366-ROCK2 staining in CCR3-stained cells. Children with FBA (n = 4); children with asthma (n = 7); mouse model, (n = 3). Representative results are shown. **P < 0.01 vs. children with FBA or mice challenged with NS.